Dr. Laurent Deluc: Associate Professor in plant genomics - Principal Investigator of the lab. In 1994, I received a B.S. degree in Plant Genetics and Molecular Sciences at the University of Bordeaux (France). One year later, I received a M.S. degree in Integrative Biology at the University of Nancy I (France). In 2004, I successfully defended my Ph.D in Plant Molecular Biology in the Food Sciences Department at the University of Bordeaux 1 (France). My Ph.D research focused on the functional validation of two regulatory proteins (MYB transcription factor) involved in the regulation of polyphenols' accumulation during grape berry development. Later this year, I joined Dr. Cushman laboratory at the University of Reno to study drought tolerance in grapevine using Affymetrix Microarrays. Two years later, still in Reno, I joined Dr. Cramer's laboratory to study the molecular mechanisms responsible for the entry of grapevine buds into endodormancy. In 2009, I was offered a position of Assistant professor in the Department of Horticulture at Oregon State University. Meanwhile, I became a core member of the Oregon Wine Research Institute wherein I initially developed a research program on different aspects of wine grape performance for improved grape and wine production in Oregon. Since then, I have extended my research interests to other aspects of plant development and tolerance to biotic and abiotic stress using other plant models such as strawberry and Brachypodium Distachyon: a plant model system for cereal crop. Functional genomics including genetic engineering, plant pathogen-interactions plant physiology, and bioinformatics are the main tools used to address the different scientific questions.
Current members of the lab
Satayanarayana Gouthu (PhD): Satyanarayana is a Senior Research Associate from India. Before going the lab, he was Post-Doctoral Scientist in Dr. Cushman Lab at the University of Reno, Nevada where he studied the protein interactomes in Arabidopsis Thaliana under stress conditions. In 2010, he joined the lab and developed several research projects focused on the regulation of ripening in grape berry. Since 2016, he has been heavily involved in the development of the microvine platform in collaboration with Dr. Mark Thomas. He has coordinated a project funded by the Oregon Wine Board and the Erath Family Foundation which the main research objective is to validate the function of a regulatory protein involved in the auxin signaling, Auxin Response Factor 4. He has developed now one new project recently funded by the American Vineyard Foundation and the Erath Family Foundation aiming at developing a DNA-Free Genome editing platform using the microvine. The long-term objective of the project is to provide the industry with transgene-free edited grapevine materials for key performance traits in grapevine production (drought tolerance, disease resistance, quality).
Landry Rossdeutsch (PhD): Landry joined the lab in September 2016 as Post-Doctoral student to develop a new research project for the lab focused on understanding the physiology of rootstock-scion interactions of grapevines. This project is a collaborative work between three researchers who are members of the Oregon Wine Research Institute (Drs. Paul Schreiner, Patty Skinkis, and Laurent Deluc). In 2015, he defended his Ph.D at the University of Bordeaux where he studied the physical and chemical mechanisms responsible for the regulation of stomatal closure under water deficit conditions. He mainly explored long-distance signaling mediated by roots by studying plant hormone and hydraulic signals on grapevines with different rootstock-scion combinations. At OSU, he is currently developing a multifaceted project with short and long-term objectives. The short-term objectives will provide grapevine physiologists and applied viticulture researchers with a better understanding of how vine vigor and nitrogen affects root/shoot coordinated growth. The long-term goal is to define which physiological and molecular mechanisms result in different growth patterns depending on the rootstock/scion combinations and whether long distance signal communication is part of the observed growing patterns of grapevine in high nitrogen soil availability in Oregon.
Mahmut Mesut Diker: Mahmut Mesut is a PhD student in plant breeding and genetics program in horticulture department. I am from Bursa locating in the northwest part of Turkey. He has completed my Bachelors and MSc degree at Suleyman Demirel University. His interests are related to genetics of grapevines and new breeding techniques. His work in the lab are focused on two research areas,. In the first one, he wants to understand the influence of the seed-derived auxin during the ripening initiating on the development of the pericarp tissues. To address this question, he is currently generating two series of transgenic lines. The first series is n3GFP and ntdTomato gene under the control of an auxin inducible promoter to characterize the auxin signaling at the gene expression level (Liao et al., 2015). The second series of transgenic lines express the ntdTomato- and Venus-Aux/IAA degradation Domain II fusion proteins under a constitutive expression and is meant to evaluate the auxin response through the degradation of the fusion proteins (Liao et al., 2015). On the second research project, he is interested in developing a CRISPR/Cas9 interference and activation pipeline at OSU to study a small class of epigenetic regulator genes (Jumomji-related demethylase). He is currently preparing different gateway vectors under under our current conditional expression system in order to understand the importance of demethylases during several transitional phases of grapevine development (fruit, shoot, and roots) and in response to environmental stresses.
Jui Chieh Lee (UG): Jui-Chieh is a senior student in the Bio-engineering program with a minor in chemistry at OSU. He was born in Taiwan in the city of Kaohsiung, in the southern tip of Taiwan, and moved here when he was 4 years old. He attended SST (school of science and technology) where he learned about the interdisciplinary field of engineering. His interest pertains in utilizing principles of synthetic biology, genomic engineering to engineer synthetic cells. His career goal is to engineer synthetic pathway neurons to create, maintain, or reinforce signals between the prosthetic limb and the brain for greater functionality and control for the user. Jui Cheh is works with Satyanarayana Gouthu on several aspects of the ARF4 project. He received the Oregon Wine Research Institute scholarship for the 2019/2020 academic year to work gene editing of the microvine.
Emanuale Chrysilla (UG): Emanuelle is a third year student in the Department of Biochemistry and Biophysics at OSU. She works on two different projects. She is assisting James and Satyanaryana in their projects and she is currently generating microvine plants expressing sgRNA-Cas9 targeting the expression of GFP expressed in microvine transgenic lines available in the lab. Her goal will be to compare the editing efficiency and off-target of the stable transformation with those from generated with the CPP-delivered Cas9 (See Satyanarayana). She is also involved in the generation of transgenic microvine lines expressed two plasmidic cassettes expressing GFP under the control of synthetic promoter containing 6 ABA-responsive Elements. The long-term goal will be to examine the spatial and temporal distribution of ABA in robots, leaves and fruits in a developmental context and in response to abiotic stress (drought).
Madesyn Samples (UG): Madesyn is a second year student in the Department of Biochemistry and Biophysics at OSU. She works on the two cloning projects.The first project aims to develop a BIFC vector to study protein-protein interaction in microvine protoplasts . The second project is to modify the Plant Gene Switch System used in the lab to contain the Red Fluorescent Protein as visual marker. The objective here is the generation of a series of a double mutant to validate the synergistic and antagonistic roles of several candidate genes identified in the lab involved in the regulation of the ripening initiation and the scion/rootstocks interaction.
Chance Lemon (UG): Chance is a Senior Undergraduate student in the Department of Biochemistry and Biophysics at OSU. He is working on two different projects in the lab with a potential applications for the Wine Industry. Both projects are based on the principle of the ectopic application of RNA-based molecules to induce RNA interference mechanisms in grapevine. The first project aims to silence the expression of genes that control the ripening initiation in grape berry. The second project focuses on the silencing of microvine (susceptibility genes) and fungal genes that could protect the grapevine against Grape Powdery Mildew. For both projects, Chance is in charge of synthesizing the Double-Stranded RNA Molecules and testing their efficiency to silence their target genes on either tissue cultured microfine plantlets or greenhouse microvine plants.
Yu Wang (G): Yu is a PhD Chinese visiting student from China Agricultural University (Beijing, China). His research interest is focused on the use of genomics data to understand the effects of microclimate on grape berry composition. Initially, he came to the lab to recieve training on bioinformatics, concentrating, on the use of computational tools aiming at building Gene Regulated Network Analyses (WGCNA and RLOwPC, Cytoscape). Additionally, he also came to improve his molecular biology and tissue culture skills. He is currently cloning one JMJ14 demethylase in order to transform it in the microvine. After his phD, he expects to come back to US for a post-doctoral research experience.
Former Members of the lab
Novia Kayfetz-Vuong (2019): Novia is a junior undergraduate visiting student from University of California Berkeley, where she is studying Biological Chemistry in the Department of Molecular & Cell Biology. She previously received an A.A. degree from Bard College at Simon’s Rock with a concentration in Chemistry and a compliment in Photography, and graduated with an achievement award from the Division of the Arts. Novia joined our lab for the summer of 2019 as part of an REEU funded by the USDA to gain experience in bioinformatics and dealing with Big Data in the agricultural sciences. She worked with Yu Wang to research differential alternative splicing events during fruit development In the future, Novia hopes to be able to take what she has learned and apply it to environmental conservation research.
Joseph Schmidt (MSc- 2015-2018): Joseph Schmidt was raised in Texas where he attended high school. In 2015, he graduated at Oregon State University with a B.S. in Horticulture, Plant Breeding & Genetics at Oregon State University. From 2015 to 2017, he went to graduate school and received a Msc in Horticulture. In the lab, he was in charge of a research project aimed to measure the accumulation dynamics of several classes of phytohormones during grape berry development, and the effects of two viticulture practices thereon. In 2018, he started his professional career as a Biological Research Technician, working for Dr. Alex Levin (SOREC) in collaboration with Deluc lab. The focus of his current work is in identifying the effects of Grape Red-Blotch-assiciated Virus (GRBaV) and irrigation on the metabolite profile of Pinot noir berries.
Karly Vial (2018-2019): Karly Vial is originally from Bend, Oregon. She transferred to Corvallis five years ago and graduated from Corvallis High School in 2016. Karly plans on majoring in biochemistry and molecular biology and is a sophomore and Oregon State University. Karly received the Oregon Wine Research Institute scholarship for the 2018/2019 academic year to work on the protoplast production in grapevine.
Tarah Gustafson (2017-2018): Tarah is a sophomore studying Biochemistry and Molecular Biology in the Honors College at OSU, with a minor in Chemistry. She is from Albany, OR. Tarah is working on a project related to Brachypodium Distachyon. The main goal of her research project is to look at how drought and nutrient deficiency (Nitrogen and Phosphorus) have an impact on the expression of genes associated with the synthesis and signaling of strigolactone, a plant hormone responsible for root shoot and root branching. She is using Brachypodium Distachyon as plant model (tillering, root growth, nutrient uptake).
Victor Puoci (2017-2018): Victor was a sophomore student studying Biochemistry and Molecular Biology in the Honors College at OSU, with a minor in Music Performance. He is from Bothell, WA and is grateful for having grown up surrounded by many opportunities for involvement science, technology, and music. Victor was awarded for the Oregon Wine Research Institute Scholarship for the 2017/2018 academic year to study protein-protein interactions in grapevine using a Yeast-Two Hybrid system.
Mandy Driskill (2017-2018): Mandie Driskill is from Springfield, Oregon. She just graduated in horticulture with an emphasis in plant breeding and genetics. Within plant breeding and genetics, her special interests are in small fruits and berries. Driskill is also minoring in business with an emphasis in entrepreneurship. Driskill’s studies have led her to two great work experiences at Oregon State University. She works as an assistant to the head breeder in the Aroma Hops Breeding Program. She is also working on grapevine genotyping in the lab. . Her passion for genetics and berries stems from her four young children. After her graduation she immediately the master in Bioinformatics at the University of Oregon.
Grace Chang (2017-2018): Grace just graduated at Oregon State University. She is from Clackamas, the suburbs of Portland, and she has lived there for the past 14 years. She is studying BioHealth Sciences with the option of Laboratory Sciences and a minor in Chemistry. She hopes to enter in the field of forensics in the future! She is working on the Yeast Two Hybrids project with Satyanarayana.
Joey Orton (2017-2018): Joey Orton is from Dallas, OR, he is a Senior in Biology with an option in genetics and a minor in chemistry. Joey assist Landry in his research project aimed to study the impact of rootstock-scion interactions on Nitrogen assimilation and uptake in relation to the shoot growth in grapevine. Joeys true passion is herpetology and hopes to pursue a doctorate degree in evolutionary ecology. Joey received the Oregon Wine Research Institute Scholarship for the 2017/2018 academic year to work on the effect of Nitrogen on grapevine vigor.
Amanda Vondras (September 2011-August 2017): Amanda graduated (BSc) from Cornell University in Spring 2011. She joined the PhD Molecular and Cellular Biology Program in Fall 2011. In the lab, Amanda studied different aspects of fruit ripening including plant physiology, metabolomics and transcriptomics. Amanda defended her PhD in August 2017. She is currently working as Post-Doctoral Student in Dario Cantu lab at UC Davis.